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dsred-rab11 wt  (Addgene inc)


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    Structured Review

    Addgene inc dsred-rab11 wt
    Dsred Rab11 Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dsred-rab11 wt/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    dsred-rab11 wt - by Bioz Stars, 2026-02
    90/100 stars

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    Image Search Results


    TMEM184B localizes to early and late endosomes in human cells. All images show HEK293T cells expressing FLAG-TMEM184B-GFP. DAPI (blue) marks the nucleus. Images represent single slices of human cells. A-D, images depicting localization of FLAG-TMEM184B-GFP to early endosomes (mCherry-Rab5, A), late endosomes (mCherry-Rab7, B), recycling endosomes (dsRed-Rab11, C), and lysosomes (LAMP1+CTSD, D). White arrows highlight overlapping puncta. Scale bars = 2 μm. n = 45-60 cells per marker, imaged over 3 sessions each. E, Fraction of merged puncta over total TMEM184B puncta area. F, Fraction of merged puncta over the total red pixel area of endosomal markers. G, Fraction of merged puncta (TMEM184B + LAMP1 + CTSD) over total TMEM184B pixel area. H, Fraction of merged puncta over the total red pixel area (LAMP1 + CTSD) of lysosomal markers. Error bars represent SEM.

    Journal: bioRxiv

    Article Title: Neuronal endolysosomal acidification relies on interactions between transmembrane protein 184B (TMEM184B) and the vesicular proton pump

    doi: 10.1101/2025.02.01.635992

    Figure Lengend Snippet: TMEM184B localizes to early and late endosomes in human cells. All images show HEK293T cells expressing FLAG-TMEM184B-GFP. DAPI (blue) marks the nucleus. Images represent single slices of human cells. A-D, images depicting localization of FLAG-TMEM184B-GFP to early endosomes (mCherry-Rab5, A), late endosomes (mCherry-Rab7, B), recycling endosomes (dsRed-Rab11, C), and lysosomes (LAMP1+CTSD, D). White arrows highlight overlapping puncta. Scale bars = 2 μm. n = 45-60 cells per marker, imaged over 3 sessions each. E, Fraction of merged puncta over total TMEM184B puncta area. F, Fraction of merged puncta over the total red pixel area of endosomal markers. G, Fraction of merged puncta (TMEM184B + LAMP1 + CTSD) over total TMEM184B pixel area. H, Fraction of merged puncta over the total red pixel area (LAMP1 + CTSD) of lysosomal markers. Error bars represent SEM.

    Article Snippet: DsRed-rab11 WT was a gift from Richard Pagano (Addgene plasmid # 12679; http://n2t.net/addgene:12679 ; RRID: Addgene_12679). pLJM1-FIRE-pHLy was a gift from Aimee Kao (Addgene plasmid # 170775; http://n2t.net/addgene:170775 ; RRID: Addgene_170775).

    Techniques: Expressing, Marker